The Sadler Lab

2024

B. O. Armijo-Galdames and J. C. Sadler, One-Pot Biosynthesis of Acetone from Waste Poly(hydroxybutyrate) ACS Sustainable Chemistry and Engineering, 2024, doi.org/10.1021/acssuschemeng.4c00357

2023

J. C. Sadler, R. C. Brewster, A. F. González, J. S. Nirkko, S. Varzandeh and S. Wallace, Overproduction of Native and Click-able Colanic Acid Slime from Engineered Escherichia coli JACS Au, 2023, 3, 378–383.

2022

J. C. Sadler, N. Swainston, M. S. Dunstan, A. Currin and D. B. Kell, SpeedyGenesXL: an Automated, High-Throughput Platform for the Preparation of Bespoke Ultralarge Variant Libraries for Directed Evolution Methods Mol. Biol., 2022, 2461, 67–83.

J. A. Dennis, J. C. Sadler, and S. Wallace, Tyramine Derivatives Catalyze the Aldol Dimerization of Butyraldehyde in the Presence of Escherichia coli ChemBioChem, 2022, 23, e202200238.

2021

J. C. Sadler,, J. A. Dennis, N. W. Johnson and S. Wallace, Microbial synthesis of vanillin from waste poly(ethylene terephthalate) Green Chem., 2021, 23, 4665–4672.

J. C. Sadler, and S. Wallace, Interfacing non-enzymatic catalysis with living microorganisms RSC Chem. Biol., 2021, 2, 1073–1083.

2020

R. J. M. Goss, J. Connolly and J. C. Sadler, SynBio: Green and clean sustainable solutions for designer pharmaceuticals, catalysis, and bioremediation Curr. Opin. Chem. Biol., 2020, 58, A1–A3.

J. C. Sadler, The Bipartisan Future of Synthetic Chemistry and Synthetic Biology ChemBioChem, 2020, 21, 3489–3491.

S. Grüschow, J. C. Sadler, P. J. Sharratt and R. J. M. Goss, Phenylalanine meta-Hydroxylase: A Single Residue Mediates Mechanistic Control of Aromatic Amino Acid Hydroxylation ChemBioChem, 2020, 21, 417–422.

2019

I. J. W. McKean, J. C. Sadler, A. Cuetos, A. Frese, L. D. Humphreys, G. Grogan, P. A. Hoskisson and G. A. Burley, S-Adenosyl Methionine Cofactor Modifications Enhance the Biocatalytic Repertoire of Small Molecule C-Alkylation Angew. Chem. Int. Ed, 2019, 58, 17583–17588.

A. Currin, J. Kwok, J. C. Sadler, E. L. Bell, N. Swainston, M. Ababi, P. Day, N. J. Turner and D. B. Kell, GeneORator: An Effective Strategy for Navigating Protein Sequence Space More Efficiently through Boolean OR-Type DNA Libraries ACS Synth. Biol., 2019, 8, 1371-1378.

2018

J. C. Sadler, A. Currin and D. B. Kell, Ultra-high throughput functional enrichment of large monoamine oxidase (MAO-N) libraries by fluorescence activated cell sorting Analyst, 2018, 143, 4747-4755.

J. C. Sadler, L. Green, N. Swainston, D. B. Kell and A. Currin, Fast and Flexible Synthesis of Combinatorial Libraries for Directed Evolution Methods Enzymol.,, 2018, 608, 59-79.

2017

J. C. Sadler, L. D. Humphreys, R. Snajdrova and G. A. Burley, A Tandem Enzymatic sp2-C-Methylation Process: Coupling in Situ S-Adenosyl-l-Methionine Formation with Methyl Transfer ChemBioChem, 2017, 18, 992-995.

J. C. Sadler, C. W. H. Chung, J. E. Mosley, G. A. Burley and L. D. Humphreys, Structural and Functional Basis of C-Methylation of Coumarin Scaffolds by NovO ACS Chem. Biol., 2017, 12, 374-379.

2016

M. Z. C. Hatit, J. C. Sadler, L. A. McLean, B. C. Whitehurst, C. P. Seath, L. D. Humphreys, R. J. Young, A. J. B. Watson and G. A. Burley, Chemoselective Sequential Click Ligations Directed by Enhanced Reactivity of an Aromatic Ynamin Org. Lett.,, 2016, 18, 1694-1697.

M. D. Benito-Alifonso, S. Tremell, J. C. Sadler, M. Berry and M. C. Galan, Imidazolium-tagged glycan probes for non-covalent labeling of live cells Chem. Commun.,, 2016, 52, 4906-4909.